Abstract
Historically, the ability to measure the velocity of phloem sap in small seedlings and plants has been technically challenging. The phloem tissues are delicate, oftenflow is blockedentirely if perturbed. Furthermore, the depth that phloem sieve tubes are located within the plant has hindered many techniques. Previously published methods have lacked the spatial and temporal resolution required for measurements in small seedlings, are usually laborious or are not suited to in vivo studies. Here we describe a rapid, high-throughput method using the fluorescent coumarin glucoside esculin as a probe to measure the phloem transport velocity in the roots of young Arabidopsis seedlings.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
