Abstract
Nitrous oxide (N2O) is a relatively stable compound, present at ∼310 nL/L in the atmosphere. It is produced predominantly by microbial reduction of nitrate (NO3−). This process, called denitrification, is the conversion of nitrate to gaseous nitrogen compounds, resulting in a product of nitrogen (N2) or nitrous oxide under most conditions. Many kinds of denitrifying bacteria have been isolated from the human oral cavity, upper respiratory tract, and alimentary tract (e.g.) (1)(2)(3)(4), including pathogens of Pseudomonas , Neisseria , and Campylobacter. Taking these studies into consideration, it is proper to assume that the concentrations of N2O in exhaled air exceed those in the atmosphere, although no studies have been published related to N2O in exhaled air. The purpose of this study is to establish an analytical method for detection of N2O in exhaled air by using gas chromatography (GC) and infrared-photoacoustic spectrometry …
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