Abstract

Anti-Brownian traps confine single molecules or particles in free solution by closed-loop feedback forces, allowing detailed characterization of photophysical and transport properties. We have recently extended this approach to tracking the particles with interferometric scattering at near infrared wavelengths. This extension allows trapping non-fluorescent particles and performing simultaneous modulated fluorescence measurements in the Interferometric Scattering Anti-Brownian ELectrokinetic (ISABEL) trap. Here we use the interferometric scattering signal in the ISABEL trap to measure the scattering cross-sections of single carboxysomes, bacterial nanocompartments involved in carbon fixation. With a core-shell model, we can calculate the total mass and internal loading of single nano-objects.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.