Abstract
N-alcohols are model general anesthetics. Past work showed that these hydrophobic molecules partition into membranes and alter miscibility transition temperatures. Past work have also found that factors that impacted rat basophilic leukemia (RBL-2H3) cell growth, such as serum starvation or cell density, also impacted miscibility transition temperatures of vesicles isolated from cells. This poster presents early investigations into if and how n-alcohols modulate cell growth. Preliminary measurements found that cell counts of RBL-2H3 cells are lower after a 24-hour incubation with 1-Butanol and greater after a 24-hour incubation with 1-Hexadecanol. To explore further, we employed a flow cytometry assay of the cell cycle using an EdU-Click reagent that incorporates into S-phase cells and DAPI stain that labels all DNA, allowing for the differentiation of a population of cells into their cell cycle phase. Using this assay, we found a dose dependent increase in cells in G0/G1 phases of the cell cycle after treatment with n-Butanol, without impact on cell size. Ongoing work is exploring the impact of other n-alcohols and possible causes for slow-down of cells through the G1 checkpoint.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
