Measurement of total free amino acids content in black tea using electronic tongue technology coupled with chemometrics

Abstract

The potential of cyclic voltammetry electronic tongue (CVET) coupled to chemometrics was investigated for measuring total free amino acids (FAA) content in black tea. Two working electrodes - glassy carbon electrode and platinum electrode were comparative and syncretic in prediction of total FAA content. Synergy interval partial least square (Si-PLS) combined with variable combination population analysis (VCPA), namely Si-VCPA-PLS, was creatively employed for searching characteristic variables selection from the whole cyclic voltammetry data set. Cyclic voltammetry signals coupled Si-VCPA-PLS model results represented its feasibility in estimating total FAA content in black tea, achieved with Rp = 0.8185 for the glassy carbon electrode; Rp = 0.8366 for the platinum electrode; and Rp = 0.8414 for their data fusion. The extracted characteristic variables were only occupying about 0.2% of the origin. This work confirmed that the cyclic voltammetry electronic tongue could be as a fast, low-cost, efficient and complementary approach for predicting total FAA content in black tea.