Abstract
An apparatus was developed for automatic measurement and to evaluate the pattern of molecular weight distribution of organic substances dissolved in water. A separation column (7.6 mm i.d., 25 mm in length) packed with Asahipak GS-220 was used. Distilled water was used as the mobile phase at flow rate of 1 ml/min, and a chromatogram was obtained by a high sensitive refractive index detector. The apparatus was regulated automatically by a system-controller. Sample size necessary for an analysis was 0.5 ml of filtrated environmental water without preconcentration, and one chromatogram was obtained in twenty minutes. Some enviromental water gave different chromatographic patterns. River water was measured by the apparatus continuously and automatically and chromatograms were obtained once an hour, which showed the change of the patterns from morning to evening. The apparatus was found to be suitable for an automatic measurement of organic substances dissolved in enviromental waters.
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