Abstract
A modified method of Tarladgis et al. (1962) and Witte et al. (1970) has been developed for quantitative determination of malondialdehyde (MDA) in fermented dairy. MDA was reacted with 2-thiobarbituric acid (TBA) in the presence of tricloracetic acid (TCA). The TBA-MDA adduct was quantitated spectrophotometric (Shimadzu UV/Vis double beam spectrophotometer, model 1700). The UV/Vis detector was set at 532 nm. Beer's law is obeyed in the concentration range of 0.186-3.724 nM/mL. The five different levels of calibration standards were prepared using 1,1,3,3-tetraethoxypropane (TEP), the precursor of malondialdehyde.
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Schedule a callMore From: Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture
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