Abstract
The diffusion coefficient of a cryoprotectant mixture in biological tissues consisting of multiple cells is measured. Obtaining reliable information on physico-chemical properties is essentilal to clearify the process of cryopreservation of biological tissues, because absorption of the cryoprotectant into the cells prior to programmed freezing is necessary to avoid overgrowth of the ice nuclei formed in the intracellular solution. For the measurement, porcine arteries are used as the test specimens, assuming a steady distribution of the concentration of the cryoprotectant mixture inside the tissue ruled by Fick's law. The diffusion coefficient of the cryoprotectant mixture in biological tissues is determined by comparing the experimental time-sequential data of the concentration of the cryoprotectant mixture, whic the analytical solution of the concentration of the cryoprotectant mixture, which can be described by an exponential curve. Values are obtained as a function of temperature. With this diffusion coefficient and membrane permeabilities, an optimum pre freezing process might be designed.
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