Measurement of Abdominal Fat by Magnetic Resonance Imaging of OLETF Rats, an Animal Model of NIDDM

Abstract

We measured abdominal fat masses (intra-abdominal visceral fat summing retroperitoneal, mesenteric, and epididymal fat and subcutaneous fat) and analyzed abdominal fat distribution of Otsuka Long-Evans Tokushima Fatty (OLETF) rats with non-insulin-dependent diabetes mellitus and control strain Long-Evans Tokushima Otsuka (LETO) rats using magnetic resonance imaging. Intra-abdominal visceral and subcutaneous fat were highly correlated with body weight both in OLETF and in LETO rats. Both intra-abdominal visceral and subcutaneous fat of OLETF rats significantly accumulated compared with those of LETO rats. Intra-abdominal visceral fat mass correlated positively with subcutaneous fat mass, and the accumulation of intra-abdominal visceral fat mass was about 3.5 times that of subcutaneous fat. Thus, obesity of OLETF rats was characterized by marked accumulation of intra-abdominal visceral fat compared with that of subcutaneous fat. Body weight and abdominal fat of OLETF rats were closely correlated with the level of total plasma glucose measured by oral glucose tolerance test. However the ratio of intra-abdominal visceral to subcutaneous fat of OLETF rats was not correlated with the level of total plasma glucose.