Abstract

Clonal cultures ofProrocentrum hoffmannianum Faust (clone 882a) were grown under optimal environmental conditions for maximal okadaic acid production. The environmental conditions of 25 °C and 86 µmol photon m-2 s-1 were used to cultivateP. hoffmannianum in a semi-continuous 36-L culture vessel with continuous cell suspension and pH control. Using these conditions, a 3-fold increase in harvestable biomass and okadaic acid content was observed when compared to batch culture techniques.

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