A novel high frequency SAWR based sensor combined with living cells for shellfish toxin quantitative determination

Abstract

Shellfish toxin can be easily found in seafood, and bring potential harm to human health. Nowadays some analytical methods are used for shellfish toxin quantitative detection. However, there are some disadvantages in these analytical methods such as time consuming, high cost, etc. Therefore, a more suitable method is in demand. In this study, a novel method using high frequency surface acoustic wave resonator (SAWR) based sensor combined with living cells was developed for shellfish toxin continuous monitoring. ECA9706 cell lines were used as the sensing elements to establish the sensor device for DSP toxin okadaic acid (OA) quantitative monitoring. The sensor device is in serial with SAWR. SAWR output frequency was recorded for detecting characterization. The OA concentration of prepared solutions could be characterized by using SAWR sensor, and good linear relationship between sensor responses and OA concentration is achieved. Liquid chromatography tandem mass spectrometry (LC–MS/MS) is used for characterization of OA content in shellfish extract. Good linear relationship between OA content and the concentration determination provided by SAWR sensor. HEK293 cells were fixed on electrode and utilized as negative control. The results showed that ECA9706 cell-based sensor had selective responses to OA. This method is promising in shellfish toxins rapid quantitative determination.