Abstract
Seedling and flag leaves of three barley cultivars were simultaneously inoculated with urediospores of barley leaf rust, race 1-2-1, and incubated at the same greenhouse bench. The inoculated leaves were harvested before urediospore formation was initiated. The volume of a large number of colonies was estimated by measuring colony area and colony depth by embedding the colony containing leaf segments into paraffin for microtome cutting. The colony volume was considerably smaller in flag leaves than in seedling leaves even in the extremely susceptible cultivar. On average the difference was about tenfold.
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