Mating in Chlamydomonas chlamydogama at Various Temperatures Under Continuous Illumination

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Trainor, Francis R. (U. Connecticut, Storrs). Mating in Chlamydomonas chlamydogama at various temperatures under continuous illumination. Amer. Jour. Bot. 47(6) : 482–484. Illus. 1960.—Mating experiments were conducted with C. chlamydogama under continuous illumination of 400 ft.-c. at temperatures of 22, 26, 30, 34 and 38°C. In 4-day experiments, low zygospore yields were obtained unless the mixed mating types were stored in darkness at 22°C. after temperature treatment. High zygospore yields, approximately 70%, were obtained at temperatures of 30 and 34°C, if followed by 24 hr. in darkness at 22°C. This yield is the same as that obtained in the controls under 12-hr. diurnal illumination at room temperature. Temperature increase probably speeds up the mechanism stimulated by nitrogen deficiency and thus evokes gametogenesis.