Abstract
Continuous affinity-recycle extraction (CARE), a continuous protein purification unit operation, has been designed to address design and optimization criteria relevant for process scale chromatographic separation of proteins. The development and application of a mathematical model describing purification in the CARE process are described. The model incorporates adsorption-desorption kinetics into material balance equations describing the operation of two well-mixed reactors operating with recycle. An accurate mathematical model of CARE has aided in its development as a new unit operation for protein purification, in the assessment of its performance tradeoffs, and in its optimization.
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