Mathematical modeling of the process of sterilizing potato explants and obtaining viable potato microclones

Abstract

The aim of the studies is obtaining optimal regime parameters of the process of decontamination of plants’ explants by investigating integral action of sterilizing agent and by modifying nutrient medium. The studies of integral influence of technological factors, which have an impact on the process of explants’ sterilization and output of viable plants, have been carried out by the method of experimental design. As a result of the analysis of literature data and searching experiments three main controllable factors, which influence on a percentage of viable potato sprouts, have been highlighted: a concentration of the antibiotic «Cefotaxime», a concentration of the cytokinin of the 6-benzylaminopurine (6-BAP) and a concentration of the commercial drug «Belizna» (the active substance is sodium hypochlorite), as output index – a percentage of viable plants. Rechtschafner design for three-factor experiment has been realized to study optimum region. The optimal values of regime parameters of the process of decontamination of explants of plants’ material have been determined on the basis of regression equations and two-dimensional sections of yield surfaces: the concentration of the antibiotic «Cefotaxime» in the nutrient medium – 335…365 mg/l, the concentration of the phytohormone 6-BAP - 0.95.1.05 mg/l, the concentration of the commercial drug «Belizna» (the active substance is sodium hypochlorite) – 2.5.2.6 %. At that the percentage of viable plants will amount to 86.0 %.