Abstract
The fiber-optic biosensor with encapsuled bioreporters use a special optical element (OE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector for on-line measurement in remote localities. The active layer of biosensor contents bioluminescent bioreporters – genetic modified cells, which are sensitive to its surrounding environment-immobilized in silica matrix on the end of OE. Bioreporters are able to react by emission of visible light (≈500 nm) – bioluminiscence reaction (BL) – in presence of specific analyte. The genetic modified bacterial stain Pseudomonas putida TVA8 was chosen as biorecognition part of biosensor producing BL in the presence of benzene, toluene, ethylbenzene and xylens (BTEX). Very low level of BL signal was detected by high-sensitive photon-counter. The sensitivity of biosensor depends on a value of detected BL and it can be very low. The signal is also affected by number of cells (light sources) immobilized into the active layer and transmission through OE. Mathematical model of OE shape was developed based on geometric optics. The numerical model is built around the MATLAB scripting language. Simulation of ray transmission was calculated for OE with different shape.
Highlights
Optical fiber biosensors as instruments for simple, rapid and continuous in-situ monitoring techniques have been intensively investigated [1,2]
Methods of geometric optic were applied for calculation of the efficiency of bioluminiscence reaction (BL) coupling into optical element (OE)
Repetitive inductions of bioluminescence of P. putida TVA8 immobilised by adsorption on modified surface of optical fiber was studied by authors [15]
Summary
Optical fiber biosensors as instruments for simple, rapid and continuous in-situ monitoring techniques have been intensively investigated [1,2]. Bioactive sensing layer (BSL) (as one of the base parts of biosensor) with transducer provides output optical signal under certain conditions. This optical signal brings information about changes in BSL caused by changes of chemical or physical properties in its close surrounding. BSL is realized by immobilizing cells - bioluminescent bioreporters (BB) - which are able produce bioluminescence (BL) in contact with a specific substance. BB represents point light sources which are immobilized in matrices from suitable material [6]. The performance of the biosensorsystem was examined as a function of following parameters: a) gel probe matrix volume, b) bacterial cell density and c) numerical aperture (NA) of the fiber core
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