Mathematical Foundation of Sparsity-Based Multi-Illumination Super-Resolution∗

Abstract

It is well-known that the resolution of a traditional optical imaging system is limited by the so-called Rayleigh limit, which is of several hundreds of nanometers. By employing fluorescence techniques, modern microscopic methods can resolve point scatterers separated much lower than the Rayleigh limit. Localization-based fluorescence subwavelength imaging techniques such as PALM and STORM can achieve a spatial resolution of several tens of nanometers. However, these techniques have limited temporal resolution as they require tens of thousands of exposures. Employing sparsity-based models and recovery algorithms is a natural way to reduce the number of exposures and hence obtain high temporal resolution. Recently, a new multi-illumination imaging technique called Brownian Excitation Amplitude Modulation microscopy (BEAM) is introduced. BEAM achieves a threefold resolution improvement by applying a compressive sensing recovery algorithm over only few frames. Motivated by BEAM, our aim in this paper is to pioneer the mathematical foundation for sparsity-based multi-illumination super-resolution. We consider several diffraction-limited images from samples exposed to different illumination patterns and recover the source by considering the sparsest solution. We estimate the minimum separation distance between point scatterers so that they could be stably recovered. By this estimation, we reveal the dependence of the resolution on the cut-off frequency of the imaging system, the SNR, the sparsity of point scatterers, and the incoherence of illumination patterns. Our theory particularly highlights the importance of the high incoherence of illumination patterns in enhancing the resolution. It also demonstrates that super-resolution can be achieved using sparsity-based multi-illumination imaging with very few frames, whereby the spatio-temporal super-resolution becomes possible.