Maternal suppplementation with conjugated linoleic acid reduce anxiety and lipid peroxidation in the offspring brain

Abstract

BackgroundMaternal consumption of fatty acids can alter neuronal membrane function, synaptic connections, and protect the brain from alterations caused by disturbances such as lipid peroxidation and anxiety in the offspring. We aimed to investigate how the maternal consumption of conjugated linoleic acid (CLA) interferes in anxiety behavior of the offspring and cerebral lipid peroxidation. MethodsThree groups were formed: control (CG) - diet without CLA; CLA1 - diet containing 1% of CLA; and CLA3 - diet containing 3% of CLA. These diets were offered to the mothers from the 7th day of gestation until the end of lactation. The following behavioral tests were used: Elevated plus maze (EPM), Open Field (OF) and Light-dark Box (LDB). Levels of malondialdehyde (MDA) and glutathione were measured in the offspring's brains. Data were analyzed by ANOVA followed by the Holm–Sidak post-test or the Kruskal–Wallis test (p < 0.05). ResultsCLA1 and CLA3 showed higher number of entries in the open arms and time spent in the central area in EPM, they translocated and ambulated more in the clear area of the LDB and presented more rearing in the OF compared to CG (p < 0.05); moreover, they presented higher concentration of glutathione and lower MDA in brain tissue (p < 0.05). LimitationsWe evaluated the effect of maternal consumption of CLA on anxiety and lipid peroxidation in rats’ offspring, but a similar study should be performed in humans. ConclusionsMaternal intake of CLA induced a decrease in the parameters of anxiety and cerebral lipid peroxidation in the offspring.