Abstract
BackgroundArtificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques. Production of pure lines by DH induction provides a new way to achieve homozygosity earlier in B.napus. Previously, the mechanism of induction, and whether the induction has obvious maternal genotypic differences or not, are not known so far.ResultsIn this study, different karyogene and cytoplasmic genotype of B.napus were pollinated with the previously reported DH inducers e.g. Y3380 and Y3560. Our study presents a fine comparison of different cytoplasmic genotypes hybridization to unravel the mechanism of DH induction. Ploidy identification, fertility and SSR marker analysis of induced F1 generation, revealed that ploidy and phenotype of the induced F1 plants were consistent with that type of maternal, rather than paternal parent. The SNP chip analysis revealed that induction efficiency of DH inducers were affected by the karyogene when the maternal cytoplasmic genotypes were the same. However, DH induction efficiency was also affected by cytoplasmic genotype when the karyogenes were same, and the offspring of the ogura cytoplasm showed high frequency inducer gene hybridization or low-frequency infiltration.ConclusionThe induction effect is influenced by the interaction between maternal karyogene and cytoplasmic genotype, and the results from the partial hybridization of progeny chromosomes indicate that the induction process may be attributed to the selective elimination of paternal chromosome. This study provides a basis for exploring the mechanism of DH inducer in B.napus, and provides new insights for utilization of inducers in molecular breeding.
Highlights
Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques
Rapeseed scientists are keen to find that is there a simpler and more efficient technique than isolated microspore culture that can quickly and efficiently obtain pure lines of B.napus? In recent years, in-vivo haploid induction line was derived from the maize Stock6 [9] and the haploid induction line mediated by the Arabidopsis CENH3 gene have been successfully used in maize [10], barley [11], and rice [12]
A recent study reported, in which an allotetraploid B.napus was crossed with an allo-octaploid rape (AAAACCCC, 2n = 8x ≈ 76) [17] and two allooctaploid rapes had induced the function of the maternal parent to produce double haploids (DH) and named as the DH induction lines in B.napus: Y3560 and Y3380 [18]
Summary
Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques. Rapeseed scientists are keen to find that is there a simpler and more efficient technique than isolated microspore culture that can quickly and efficiently obtain pure lines of B.napus? SSR molecular markers, plant ploidy, and morphological identification revealed that a higher proportion of plants in the F1 generation were similar to the maternal parent and induction efficiency ranges from 34.09% ~ 98.66%. What accounts for such a huge difference in induction efficiency? This study lays the foundation for the application of the DH lines and contributes to the understanding of maternal karyotype and cytoplasmic genotype effects
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