Maternal High-Fat Diet during Pregnancy and Lactation Influences Obestatin and Ghrelin Concentrations in Milk and Plasma of Wistar Rat Dams and Their Offspring.

Monika Słupecka,Katarzyna Romanowicz,Jarosław Woliński

doi:10.1155/2016/5739763

Monika Słupecka, Katarzyna Romanowicz + Show 1 more

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https://doi.org/10.1155/2016/5739763

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Abstract

The study aims to establish the effect of a maternal high-fat diet on obestatin concentration, total ghrelin, and ghrelin/obestatin ratio during pregnancy and lactation of Wistar rats and their offspring in the first 21 days of life. On the mating day, females were randomly allocated and fed either a high-fat diet (30% of fat; HF) or breeding diet (5% fat; BD) till the 21st day of lactation. Hormones were analyzed in the blood plasma and milk of rat dams as well as in the blood plasma of their offspring. HF resulted in a significant decrease in obestatin level on the 14th day of lactation and elevation on the 21st day. Plasma obestatin in HFD offspring was significantly higher than in BD ones. HF diet did not significantly affect dam plasma ghrelin until the 21st day of lactation. The ghrelin concentrations in milk after both diets were significantly lower than in blood plasma. Milk ghrelin in HF dams was significantly higher than in the BD ones. Plasma ghrelin from HF offspring was significantly higher than that from BD dams. Our results demonstrate that a maternal HF diet during pregnancy and lactation influences ghrelin and obestatin level in both dams and their offspring.

Highlights

Development and growth during the early life period are influenced by maternal health and diet composition [1]
Feeding dam rats with high-fat diet during pregnancy and lactation resulted in significant increase in body weight and plasma triglycerides of their offspring (HFO) starting from the 14th day of life as compared to control pups (BDO) (Table 2(b))
The positive correlation was observed between the body weight of breeding diet (BD) dams and plasma blood obestatin concentration in the 21st day of lactation (r = 0.976, p = 0.002), whereas in the HF dams we found a negative correlation (r = −0.881, p = 0.010) (Table 3(a))

Summary

Introduction

Development and growth during the early life period are influenced by maternal health and diet composition [1]. Evidence from both epidemiological and animal model studies indicates that maternal diet influences metabolic status and plays a crucial role in the development of metabolic functions in offspring and their susceptibility to metabolic diseases in adulthood. Studies in rats show that a high-fat diet during pregnancy and lactation has a marked impact on offspring body composition, risk of metabolic syndrome [2], and the development of obesity in both early life and adulthood [3, 4]. Ghrelin and obestatin are gastrointestinal peptides involved in the regulation of metabolic functions in rodents and humans.

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