Mass spectrometry based methods for the enzyme substrate screening and kinetics analysis

Abstract

Revealing the relationship between the enzyme and substrate could provide insights into signaling networks. Mass spectrometry has been widely applied for the large scale interrogation of biological systems in proteomics, and also has taken an important role in determination of enzyme activities and screening of enzyme inhibitors. In this review, we introduced the recent advance in the mass spectrometry based methods for enzyme substrate screening and enzyme kinetics analysis, including in vitro substrate screening using protein-derived library and peptide-derived library, in vivo substrate screening and high-throughput kinetics analysis, and then gave our opinions on the outlook of mass spectrometry in the study of enzyme kinetics in complex system.