Abstract
Since mass loss is a fundamental limitation in electron microscopy and microanalysis of biological materials it is very valuable to understand the effect of specimen temperature on the mass loss process. An electron energy loss spectrometer has been added to the Duke University Cryomicroscope (fig. 1) to allow a general study of radiation damage effects in specimens held at liquid helium temperature.The reduction of specimen mass is measured by recording a long series of electron energy loss spectra with a Tracor-Northern TN-5400 analyzer, using the region integration feature of the analyzer to report the number of counts in the zero-loss and inelastic scattering regions of the spectrum.
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