Abstract
AbstractThe effect of ATP on the binding of Na, K, Mg and Ca ions by rat liver microsomes was investigated. Addition of increasing amounts of ATP to a suspension of microsomes in an ionic medium containing these cations caused a marked decrease in divalent cation binding and a concomitant increase in monovalent cation binding by the microsomes. The strong nonbiological complexing agent EDTA produced qualitatively similar but quantitatively greater changes in binding. Although ATP‐dependent binding of monovalent cations has been interpreted by other investigators in terms of an ATPase system and the formation of a hypothetical monovalent cation binding intermediate, the present data demonstrate that the role of ATP may also be reasonably explained in terms of mass law interactions of the various known solute species and fixed membrane binding sites involved.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
