Markerfreie Karyotypisierung von Chromosomen durch spektrales Imaging

Abstract

Karyotyping is currently based on staining techniques. These methods lack reproducibility, are time-consuming and require complex expert knowledge. This paper introduces a new marker-free, fully automated and reliable screening procedure for chromosomes. The method is based on multimodal, spatially resolved spectroscopy combined with multivariate data analysis and can easily be integrated into a standard light microscope.