Abstract
Polypeptide pattern alterations during somatic embryogenesis were investigated using callus cultures of two Pisum sativum genotypes. Both genotypes show the formation of two different callus lines from the same explant after six to eight weeks in culture: a nodular yellowish callus line, which forms somatic embryoids in suspension cultures (e(+)) and a white compact callus line with no regenerative capacity (e(-)). The cytosol proteins of the two different callus lines were separated in a semi-preparative two-dimensional system and the polypeptide patterns were compared. Two protein bands were found (P1: Mr=45000 D, pI=7.0-7.1; P2: Mr=7000 D, pI=<4.5), which were characteristic of the putatively embryogenic (e(+)) callus line in all tissues investigated (two genotypes × two explant sources). These proteins found in nodular (e(+)) pea cultures are very similar to two proteins found in Daucus carota suspension cultures preceding the formation of somatic embryos.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
