Occurrence of somatic embryoids in suspension callus cultures initiated from immature haploid wheat embryos.

Abstract

Wheat callus and suspension cultures were initiated from 22 immature haploid embryos obtained from intergeneric crosses with Hordeum bulbosum L. using an F1 wheat hybrid. Two different callus lines were induced after 10 weeks of suspension culture. One line consisted of compact and friable callus clusters which proliferated rapidly in a liquid medium. A few of the calli transferred onto the solid medium were regenerated to green plants through somatic embryogenesis. The other line, growing slowly, consisted of somatic embryoids which were successfully regenerated to green plantlets, but in most of the cases failed to undergo further development. The difference in the behaviour of wheat suspension cultures was discussed.