Marine-Sulfated Polysaccharides Extracts Exhibit Contrasted Time-Dependent Immunomodulatory and Antiviral Properties on Porcine Monocytes and Alveolar Macrophages

Abstract

Simple SummaryAlgal extracts have a real potential in terms of animal health, strengthening the interest in this natural resource. In pigs, respiratory complex syndrome significantly alters the wellbeing of the animals and threatens the economical sustainability of the sector. In the current study, we assessed various marine-sulfated polysaccharides (MSP®) extracts on two relevant cell populations in pigs, i.e., porcine monocytes and alveolar macrophages. Then, we analyzed the impact of the extracts on the infections of the cells by two important viruses. A modulation of the inflammatory response as well as some inhibitions of viral replication were observed. The type of effect observed was dependent on the extract, the experiment set-up, and the virus. The results obtained prompt us to further decipher the effects of algal extracts on the porcine health and open the door to future experiments, particularly in vivo experiments.Porcine respiratory complex syndrome has a strong economic impact on the swine breeding sector, as well as a clear repercussion on the wellbeing of the animals, leading to overuse of antimicrobial molecules. Algal extracts used in short-term treatments are empirically recognized by farmers as having a positive effect on pigs’ health, however, their mechanisms of action are not well known and more research is needed. Herein we studied the short and median term impact of three algal extracts, in vitro, on the pro-inflammatory and antiviral responses of porcine primary blood monocytes and alveolar macrophages, as well as the susceptibility of the treated cells to infection by Porcine Respiratory and Reproductive Syndrome Virus (PRRSV) and the Aujeszky’s Disease Virus (ADV). All extracts presented a pro-inflammatory short-term effect, associated for two of them, with an inhibition of the PRRSV replication. Conversely, the three extracts presented an anti-inflammatory median term effect, with no impact on PRRSV replication. The observed immune modulation prompts us to test, in vivo, the anti-PRRSV action of algal extracts and strengthen the interest for this natural resource.