Abstract
Background: Free radical generation has been proved to be responsible for various cellular diseases. It is necessary to combat free radicals using antioxidants derived from natural sources. Objective: The objective of this study is to evaluate the antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 isolated from mangrove sediment soil collected from Pichavaram, Tamil Nadu, India. Methods: The antioxidant potential of pyrrolo [1, 2-A] pyrazine-1, 4-dione, hexahydro-3-(2-methylpropyl) (diketopiperazine) extracted from Streptomyces sp. VITMK1 was studied using reducing power assay. The scavenging of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and nitric oxide (NO) radical by the compound was also studied. The cytotoxic activity of the compound on RAW 264.7 macrophage cell line was studied using MTT cell viability assay. Results: The compound exhibited strong DPPH radical scavenging activity (72.48±0.32% at 500 µg/mL) and NO radical scavenging activity (73.03±1.02% at 500 µg/mL). MTT cell viability assay revealed that the compound exhibited concentration-dependent cell viability and was observed to be 92% at 125 µg/mL concentration. Conclusion: The antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 can be probed further to establish its radical scavenging activity.
Highlights
Reactive oxygen species (ROS) are a group of free radicals that are derived from oxygen
The antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp
This study reports the antioxidant activity of a diketopiperazine compound, Pyrrolo [1, 2-A] pyrazine-1, 4-dione, hexahydro-3-(2methylpropyl)- extracted from Streptomyces sp
Summary
Reactive oxygen species (ROS) are a group of free radicals that are derived from oxygen. Human cells have a natural mechanism aided by enzymes superoxide dismutase, catalase and glutathione peroxidase to neutralize the free radicals thereby protecting the cells [4]. They would have developed specific defense mechanism to combat oxidative stress in their extreme conditions [9] They could serve as the natural source for novel antioxidants. Actinomycetes are a group of gram positive unicellular bacteria that have a high G+C content They are known for their ability to produce secondary metabolites with a broad spectrum of biological activity such as antimicrobial, antitumor, immunosuppressive, antioxidant, enzyme inhibitory and others [10]. It is necessary to combat free radicals using antioxidants derived from natural sources
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