Mapping PERIOD-immunoreactive cells with neurons relevant to photoperiodic response in the bean bug Riptortus pedestris.

Abstract

Circadian clock genes are involved in photoperiodic responses in many insects; however, there is a lack of understanding in the neural pathways that process photoperiodic information involving circadian clock cells. PERIOD-immunohistochemistry was conducted in the bean bug Riptortus pedestris to localise clock cells and their anatomical relationship with other brain neurons necessary for the photoperiodic response. PERIOD-immunoreactive cells were found in the six brain regions. In the optic lobe, two cell groups called lateral neuron lateral (LNl) and lateral neuron medial (LNm), were labelled anterior medial to the medulla and lobula, respectively. In the protocerebrum of the central brain, dorsal neuron (Prd), posterior neuron (Prp), and antennal lobe posterior neuron (pAL) were found. In the deutocerebrum, antennal lobe local neurons (ALln) were detected. Double immunohistochemistry revealed that PERIOD and serotonin were not co-localised. Furthermore, pigment-dispersing factor-immunoreactive neurons and anterior lobula neurons essential for R. pedestris photoperiodic response were not PERIOD immunopositive. LNl cells were located in the vicinity of the pigment-dispersing factor immunoreactive cells at the anterior base of the medulla. LNm cells were located close to the somata of the anterior lobula neurons. Fibres from the anterior lobula neurons and pigment-dispersing factor-immunoreactive neurons had contacts at the anterior base of the medulla. It is suggested that LNl cells work as clock cells involved in the photoperiodic response and the region at the medulla anterior base serves as a hub to receive photic and clock information relevant to the photoperiodic clock in R. pedestris.