Abstract
We use a pair of orthogonal tracking beams to track and analyze the Brownian motion of a micro-particle or a living cell trapped in optical tweezers to map the three-dimensional optical force field on these samples. For optical trapping with λ = 532nm, NA = 0.85, and total power = 100mW, the axial and the transverse spring constants are around 1x10-8N/m and 2x10-8N/m, respectively for a silica particle (diameter = 2.58 μm), and 3x10-8N/m and 2x10-7N/m, respectively for a Chinese hamster ovary cell (diameter ∼15 μm).
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