Abstract
Mapping of mutant and other genes in yeast to sites on a particular chromosome involves techniques which have been described already in this volume. Most of the procedure involves crossing the yeast strain carrying the unmapped gene(s) to a strain carrying mapped markers. The general approach includes tetrad analysis of the various crosses, random spore analysis if tetrad analysis is not possible, trisomic analysis (for assigning a gene to a particular chromosome), and mitotic mapping methods including mitotic crossing-over (induced by UV Hght or ionizing radiation or alkylating agents) and mitotic gene conversion, and chromosome loss and chromosome transfer. Fine-structure mapping is used for intragenic maps, and is done by X-ray treatment and treatment with recombinogenic agents, followed by meiotic and mitotic analysis, or, preferably, by deletion mapping.
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