Mapping and characterization of a quantitative trait locus resistance to the brown planthopper in the rice variety IR64

Meng Yang,Yongfu Qiu,Wan Shu,Liuhui Yan,Ling Cheng,Xinyi Wang

doi:10.1186/s41065-019-0098-4

Meng Yang, Yongfu Qiu + Show 4 more

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https://doi.org/10.1186/s41065-019-0098-4

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Abstract

BackgroundRice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. Notably, host-plant resistance was proved to be one of the effective ways to manage the pest. And stronger virulence will probably emergence when continuous use of insecticides. Therefore, more resistance genes with different resistance mechanisms were needed to be detected and then applied in the rice breeding practice.ResultsResistance genes in the rice variety IR64 were evaluated considering the seedling bulk test and seedling survival rate. As a result, a locus with a large LOD score of 7.23 was found between markers RM302 and YM35 on chromosome 1. The locus explained 36.9% of phenotypic variation and was tentatively denominated Bph37. Moreover, Bph1 was detected to be harbored by the markers RM28366 and RM463, and had the largest LOD score of 2.08, explaining 7.7% of phenotypic variance in the same mapping population. Finally, the preliminary-near-isogenic-lines (pre-NILs) carrying Bph37 exhibited significant tolerance to the insects. But no antibiotic or antixenotic effects were observed in the resistant plants when infested with the insects.ConclusionsWe mapped one major BPH resistance gene Bph37 in consideration of seedling survival rate and the resistance lines showed tolerance to BPH. The detected gene should be beneficial for understanding the resistance mechanism of rice to BPH and for insect-resistance rice breeding programs.

Highlights

IntroductionRice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions
Rice planthoppers was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions
brown planthopper (BPH) resistance and tolerance evaluation and genetic analysis almost 20 years passed, we still found that the rice variety IR64 presents moderate resistance to the BPH insects collected from the rice field of Nanning, Guangxi

Summary

Introduction

Rice planthoppers (main brown planthopper, Nilaparvata lugens Stål; BPH) was one of substantial threats to Asia rice production as its serious destruction and difficulties in control under field conditions. The brown planthopper (BPH), Nilaparvata lugens (Stål), one of the most devastating insect pests of rice (Oryza sativa L.), occurs widely in South, Southeast, and East Asia, as well as in the South Pacific islands and Australia. This insect uses its stylet to pierce the leaf sheath phloem sap and assimilate nutrients such as sucrose, amino acids, potassium, and ATP in the vascular bundle [14]. It can transmit viruses, such as grassy stunt and Plants have evolved various strategies to adapt to the external environment.

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