Mannan endo-1,4-\u03b2-mannosidase from Kitasatospora sp. isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

Nanik Rahmani,Jaemin Lee,Bambang Prasetya,Satoko Niimi-Nakamura,Yopi Yopi,Puspita Lisdiyanti,Hana Matsumoto,Chiaki Ogino,Akihiko Kondo,Prihardi Kahar,Norimasa Kashiwagi

doi:10.1186/s13568-017-0401-6

Abstract

Mannan endo-1,4-β-mannosidase (commonly known as β-mannanase) catalyzes a random cleavage of the β-d-1,4-mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocellulose biomass, as well as in production of mannooligosaccharides (MOS) for applications in feed and food industries. We aimed to obtain a β-mannanase, for such mannan polymer utilization, from actinomycetes strains isolated in Indonesia. Strains exhibiting high mannanase activity were screened, and one strain belonging to the genus Kitasatospora was selected. We obtained a β-mannanase from this strain, and an amino acid sequence of this Kitasatospora β-mannanase showed a 58–71% similarity with the amino acid sequences of Streptomyces β-mannanases. The Kitasatospora β-mannanase showed a significant level of activity (944 U/mg) against locust bean gum (0.5% w/v) and a potential for oligosaccharide production from various mannan polymers. The β-mannanase might be beneficial particularly in the enzymatic production of MOS for applications of mannan utilization.

Highlights

Mannan endo-1,4-β-mannosidase [EC 3.2.1.78] (β-mannanase) is used to catalyze a random cleavage of the β-d-1,4-mannopyranosyl linkage (McCleary and Matheson 1986). β-Mannanase is produced by various organisms, such as bacteria, yeasts, and fungi (Dhawan and Kaur 2007)
After cloning a β-mannanase gene from this strain, we found that the enzyme demonstrated the release of various types of oligosaccharides from the mannan polymers, indicating its beneficial potential in the production of MOS from raw biomass
Materials, and chemicals The actinomycetes were obtained from Biotechnology Culture Collection (BTCC), Indonesian Institute of Sciences (LIPI)

Summary

Introduction

Mannan endo-1,4-β-mannosidase [EC 3.2.1.78] (β-mannanase) is used to catalyze a random cleavage of the β-d-1,4-mannopyranosyl linkage (McCleary and Matheson 1986). β-Mannanase is produced by various organisms, such as bacteria, yeasts, and fungi (Dhawan and Kaur 2007). Mannan endo-1,4-β-mannosidase [EC 3.2.1.78] (β-mannanase) is used to catalyze a random cleavage of the β-d-1,4-mannopyranosyl linkage (McCleary and Matheson 1986). Β-Mannanase is produced by various organisms, such as bacteria, yeasts, and fungi (Dhawan and Kaur 2007). Various species of β-mannanase have been cloned and characterized from these organisms (Yamabhai et al 2016). Mannan polymers are widely distributed in plants as part of the hemicellulose fraction in hardwoods, softwoods, seeds of leguminous plants, and beans (Dhawan and Kaur 2007). The use of β-mannanase is one method to the extensive degradation of these mannan polymers for application in biofuel production from lignocellulose biomass (Ishii et al 2016), as well as in production of mannooligosaccharides (MOS) (Yamabhai et al 2016). Most of the MOS products are derived from sugar polymers present in the cell wall of Saccharomyces cerevisiae

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