Manganese Deficiency and Toxicity Effects on Photosynthesis, Chlorophyll, and Transpiration in Wheat1

Abstract

Wheat (Triticum aestivum L.) production areas are expanding to soils low in available Mn in southern Georgia and to soils high in available Mn in northern Georgia. Useful data on Mn deficient and toxic levels in leaf tissue for top growth and physiological processes in wheat are needed to evaluate growth processes limiting wheat production. The objectives of this study were to correlate Mn deficiency and toxicity levels with top dry weight production, photosynthesis, chlorophyll concentration, and transpiration. Wheat plants were grown in 10 experimental concentrations ranging from 0.09 μM to 9.1 mM in nutrient solution. Photosynthesis and transpriration measurements were made on blade 1 of plants grown in the greanhouse. Total chlorophyll and Mn concentrations were determined on the experimental tissue after freeze‐drying. Foliar dry weights on a per pot (15 plants) basis were also determined. Severe Mn deficiency depressed top dry weight, photosynthesis, and total chlorophyll concentration, but transpiration was unaffected. Mild Mn toxicity reduced top dry weight but not photosynthesis, total chlorophyll concentration, or transpiration. Critical Mn deficiency levels for reducing top dry weight and apparent photosynthesis rates were similar, namely 0.23 and 0.30 mmol kg−1, repectively. Growth reduction from Mn deficiency is possibly due to reduced photosynthesis. Minimum Mn requirements for chlorophyll (0.14 mmol kg−1) is about one‐half of that for top production. The critical Mn deficiency level for transpiration could not be determined. The critical Mn toxicity level for top dry weight occurred at 7 mmol kg−1. Concentrations of 19, 19, and 24 mmol kg−1 were required for the depression of photosynthesis, total chlorophyll concentration, and transpiration, respectively. Maximum growth and yield are expected when the range of Mn concentration in blade 1 is 0.23 to 7 mmol kg−1. The critical levels are prelimilinary values to be used in evaluating Mn status of field‐grown wheat for possible deficiency and toxicity.