Mandelstam–Brillouin Light Scattering in Bovine Serum Albumin Solutions with Different Concentrations in the Vicinity of Thermal Denaturation

Abstract

Phase transformations of bovine serum albumin (BSA) are investigated using the Brilluoin light scattering in the temperature range from 300 to 380 K in solutions with concentrations of 50 and 5 mg/mL. As the reference sequence, the sequence of phase transformations in a concentrated (100 mg/mL) BSA solution are used for analyzing the results. It is shown that the sequence of BSA phase transformations is modified upon a decrease in the concentration. For example, in the vicinity of denaturation, the region with fibrilform protein aggregates disappears, and the properties of gel-type high-temperature phase change.