Abstract
Detection of antibiotics in the blood is necessary for characterizing their common or individual pharmacokinetics. This has increased the need in rapid detection techniques, such as lateral flow immunoassay, for the on-site control of antibiotics. The present study characterized factors influencing the analytical parameters of lateral flow immunoassay to increase its sensitivity for detecting tetracycline in human serum samples. Assay sensitivity was increased by altering the concentrations of immunoreagents and surfactant and the number of interaction stages in the assay with indirect labeling a specific antibody. The optimal assay conditions reduced the limit of visual detection of tetracycline from 100 to 10 ng/mL. The developed assay allowed us to detect tetracycline in both two-fold diluted and undiluted human serum samples within 15 min. Our results suggest that the developed assay can be used to screen patients under antibiotic treatment.
Highlights
Appropriate antibiotics for medication[7]
8 mL gold nanoparticle solution was added to 150 μLaMAb solution in a glass flask, and the mixture was incubated with stirring at room temperature for 45 min
Development of lateral flow immunoassay (LFIA) involving the indirect labeling of antibody
Summary
Appropriate antibiotics for medication[7]. Adequate dosage of antibiotics is important because it decreases the intensity of side effects and affects the ability of bacteria to develop antibiotic resistance[8, 9]. Problems concerning the personalization of medicine are actual, and development of personalized medicine is important[11, 12] These factors highlight the need to control Tet level in the serum of humans under treatment. This can be achieved using different methods. Many developments have been described for the rapid analysis of different analytes in serum samples[18,19,20]. The first method is a traditional method involving the direct adsorption of an antibody on the surface of gold nanoparticles. Previous studies have shown that the direct immobilization of a specific antibody on the surface of gold nanoparticles results in the inefficient binding of the target analyte to the conjugate without decreasing an analytical signal 21. The present study investigated the most efficient method for performing the immunochromatographic detection of Tet with indirect labeling
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