Mammalian cell lines used in bioprocessing

Abstract

A various number of expressions and host systems are used in biologics manufacturing. The most commonly preferred systems are based on bacteria, yeast, mammalian cells, insect cells, and transgenic animals. A wide range of molecules, including insulin, mAbs, vaccines, and recombinant proteins, are produced using different host systems. Because of several reasons impacting the product quality and yield, mammalian cells are utilized. Moreover, mammalian cells are generally used in virus-based vaccine manufacturing. Chinese Hamster Ovary (CHO) is the most widely used cell line for high yield stable recombinant protein production, while Human Embryonic Kidney (HEK) is favoured for transient transfection low yield protein manufacturing, viral-based vaccine and gene and cell therapy-related vector production. Other mammalian cell lines such as NSO, Sp2.0, Vero, MRC-5 and PerC.6 are also used in both recombinant protein and virus productions. Multiple modifications are carried out on industrial cell lines to make them more suitable for high yield and high-quality protein production. Thanks to these alterations, high productivity and quality levels are achieved in the biotechnology industry.