Abstract
BackgroundMallotus philippinensis Muell. Arg (MP, Euphorbiaceae) are widely distributed perennial shrub or small tree in tropical and subtropical region in outer Himalayas regions. Since, Mallotus philippinensis have been shown to have a number of medicinal values. Hence our present study was to investigate the healing potential of fruit extract in rat wound models.MethodsThe study includes acute toxicity and wound healing potential of 50% ethanol extract of MP fruit glandular hair (MPE). MPE (200 mg/kg) was administered orally, once daily for 10 days (incision and dead space wound) and 22 days (excision wound). MPE was found safe when given to rats upto 10 times of optimal effective dose. Wound breaking strength (WBS) in Incision wound and rate of contraction, period of epithelization and scar area in Excision wound were evaluated. Granulation tissue free radicals (nitric oxide and lipid peroxidation), antioxidants (catalase, superoxide dismutase, and reduced glutathione), acute inflammatory marker (myeloperoxidase), connective tissue markers (hydroxyproline, hexosamine, and hexuronic acid), and deep connective tissue histology were studied in Dead space wound.ResultsMPE significantly increased WBS and enhanced wound contraction, and decreased both epithelization period and scar area compared with control group. MPE was found to decrease free radicals (50.8 to 55.2%, P<0.001) and myeloperoxidase (44.0%, P<0.001) but enhanced antioxidants (41.1 to 54.5%, P<0.05 to P<0.001) and connective tissue markers (39.5 to 67.3%, P<0.05 to P<0.01). Histopathological evaluation revealed more density of collagen formation with minimal inflammatory cells in deeper tissues.ConclusionThus, the study revealed Mallotus philippinensis fruit hair extract, safe and effective in wound healing and the healing effects seemed to be due to decrease in free radical generated tissue damage, promoting effects on antioxidant status and faster collagen deposition as evidenced biochemically and histology.
Highlights
We have studied that Mallotus philippinensis (MP) fruit extract possessed good anti-inflammatory and analgesic activity which can be important for the healing effect of MP
During the day of observation period, the animals were observed no significant sign of toxicity, adverse pharmacological effects or abnormal behavior
This result may indicate that Mallotus philippinensis fruit extract has no acute toxicity
Summary
We have studied that MP fruit extract possessed good anti-inflammatory and analgesic activity which can be important for the healing effect of MP. We studied the ethanol extract of MP for its wound healing activity This plant is employed in herpetic ringworm, scabies and other parasitic skin diseases. Protocatechuic acid has been shown to promote the migration and proliferation of adipose tissue-derived stromal cells (ADSCs) in vitro. These data suggest that the components of MP may provide new therapeutic options for regenerative medicine and could remodel wounded tissues [10].
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