Abstract
Mass spectrometry is a modern physicochemical method of analysis that allows for a qualitative and quantitative analysis of the composition of a substancebased on the preliminary ionization of its constituent atoms or molecules. One of the new methods of ionization, thanks to which the mass spectrometric study of macromolecules has become widespread, is matrix-activated laser desorption / ionization (MALDI), which is a pulsed laser irradiation of the substance under study mixed with a matrix.Тhe article presents modern data on the use of the MALDI mass- spectrometry method for conducting genus and species-specific identification of microorganisms in the practice of diagnostic laboratories. The advantages of MALDI-TOF identification in comparison with other methods of microbiota research are considered.The place of mass spectrometry in the system of laboratory diagnostics of various pathogens is indicated.
Highlights
National Medical University named after O.O. Bogomolets
quantitative analysis of the composition of a substance based on the preliminary ionization of its constituent atoms or molecules
a pulsed laser irradiation of the substance under study mixed with a matrix
Summary
Трудности лабораторной внутриродовой и внутривидовой дифференциации бактерий связаны с наличием общих биохимических свойств, морфологических, тинкториальных характеристик, присутствием родоспецифических и перекрестно реагирующих антигенов. Y. pestis была однозначно идентифицирована на видовом уровне, а MALDI-TOF была успешно применена для дифференциации трех биотипов. V.Ryzhov et al [39] провели MALDI-TOF массспектрометрический анализ 14 микроорганизмов группы Bacillus cereus. L.Ferreira et al [20] провели MALDI-TOF MS исследование 131 клинического изолята рода Brucella, включая виды B. melitensis, B. abortus, B. suis, предварительно идентифицированных традиционными методами и ПЦР. B.Schilling et al [41] применили метод вакуумной MALDI масс-спектрометрии (vMALDI) для получения масс-спектров и определения структуры липида, а трех видов рода Francisella (F. tularensis, F. novicida и F. philomiragia). A.Silipo et al [45] провели MALDI анализ вторичной структуры жирных кислот, входящих в состав липина, а для видов E. coli, K. pneumoniae, K. oxytoca, P. reactans и B. caryophylli. J.Gidden et al [23] осуществили MALDI-TOF масс-спектрометрический анализ липидов E. coli и B. subtilis. Aureus [21] и шига-токсина E. coli O157 [19]
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