Abstract

In 1996, independent publications demonstrated the application of MALDI-TOF MS for microorganism identification using whole-cell profile mass spectra. As these were characteristic for distinct bacterial and fungal species, they could be used as a molecular fingerprint. Subsequently, sample preparation, data evaluation with bioinformatics and further aspects of the technology were further improved. Ease-of-use, rapidity and accuracy of this technology finally led to the implementation of MALDI-TOF MS into routine analytical work, particularly in clinical microbiology.

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