Abstract
Mass spectrometry (MS), a core technology for proteomics and metabolomics, is currently being developed for clinical applications. The identification of microorganisms in clinical samples using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is a representative MS-based proteomics application that is relevant to daily clinical practice. This technology has the advantages of convenience, speed, and accuracy when compared with conventional biochemical methods. MALDI-TOF MS can shorten the time used for microbial identification by about 1 day in routine workflows. Sample preparation from microbial colonies has been improved, increasing the accuracy and speed of identification. MALDI-TOF MS is also used for testing blood, cerebrospinal fluid, and urine, because it can directly identify the microorganisms in these liquid samples without prior culture or subculture. Thus, MALDI-TOF MS has the potential to improve patient prognosis and decrease the length of hospitalization and is therefore currently considered an essential tool in clinical microbiology. Furthermore, MALDI-TOF MS is currently being combined with other technologies, such as flow cytometry, to expand the scope of clinical applications.
Highlights
Mass spectrometry (MS) applications have expanded significantly in recent years [1]
The MALDI method was combined with time-of-flight mass spectrometry (TOF MS) to become MALDI-TOF MS, which quickly became a widely used technique
If no corresponding species-level identification could be obtained from the biochemical typing and mass spectrometry fingerprinting, full-length 16S rRNA gene sequences were acquired [43]. 16S rRNA gene analyses were used for species-level delineation based on assigning the closest type strain
Summary
Mass spectrometry (MS) applications have expanded significantly in recent years [1]. After years of efforts by concerned organizations and academic societies, MS was officially approved by the Japanese. Is one of the most widely used MS methods applied to the identification of microorganisms in clinical samples [5,6,7,8,9,10,11,12,13] This new application has proven useful in the diagnosis and treatment of infectious diseases that require a swift response. Identifying microorganisms using MALDI-TOF MS is convenient, rapid, and accurate while being cost-effective. This method has revolutionized microbial identification (ID) in clinical microbiology laboratories [14,15]. MS to identify microorganisms, as well as its use in combination with other methods
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