Abstract
BackgroundThe pathogenesis of multiple myeloma involves complex genetic and epigenetic events. This study aimed to investigate the role and clinical relevance of the long non-coding RNA (lncRNA), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in multiple myeloma.MethodsBone marrow mononuclear cells were collected for analysis. The samples of multiple myeloma were taken from 45 patients at diagnosis, 61 post-treatment, and 18 who relapsed or had progression. Control samples were collected from 20 healthy individuals. Real-time quantitative reverse transcription polymerase chain reactions were performed to evaluate the expression of MALAT1. The clinical relevance of MALAT1 expression was also explored.ResultsMALAT1 was overexpressed in the newly diagnosed patients compared with post-treatment patients (mean ∆CT: -5.54 ± 0.16 vs. -3.84 ± 0.09, 3.25-fold change; p < 0.001) and healthy individuals (mean ∆CT: -5.54 ± 0.16 vs. -3.95 ± 0.21, 3.01-fold change; p < 0.001). The expression of MALAT1 strongly correlated with disease status, and the magnitude of change in MALAT1 post-treatment had prognostic relevance. The patients with early progression had a significantly smaller change in MALAT1 after treatment (mean ∆CT change: 1.26 ± 1.06 vs. 2.09 ± 0.79, p = 0.011). A cut-off value of the change in MALAT1 (∆CT change: 1.5) was obtained, and the patients with a greater decrease in MALAT1 (difference in ∆CT >1.5) had significantly longer progression-free survival compared with the patients with a smaller MALAT1 change (24 months vs. 11 months; p = 0.001). For the post-treatment patients, the risk of early progression could be predicted using this cut-off value.ConclusionsMALAT1 was overexpressed in patients with myeloma and may play a role in its pathogenesis. In addition, MALAT1 may serve as a molecular predictor of early progression.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2407-14-809) contains supplementary material, which is available to authorized users.
Highlights
The pathogenesis of multiple myeloma involves complex genetic and epigenetic events
Multiple myeloma is a hematological malignancy characterized by abnormal proliferation of monoclonal plasma cells in bone marrow leading to various end-organ damage including anemia, hypercalcemia, renal insufficiency and osteolytic bone disease [1]
The long non-coding RNA (lncRNA) have been highly conserved throughout mammalian evolution including in humans, and they have been shown to be aberrantly expressed in cancer tissue and to be involved in oncogenic or tumor suppressive processes [13]
Summary
This study aimed to investigate the role and clinical relevance of the long non-coding RNA (lncRNA), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in multiple myeloma. The lncRNAs have been highly conserved throughout mammalian evolution including in humans, and they have been shown to be aberrantly expressed in cancer tissue and to be involved in oncogenic or tumor suppressive processes [13]. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is one of the few biologically well-studied lncRNAs, and is located on chromosome 11 (11q13.1). This lncRNA is highly conserved in mammals and is more than 8000 nucleotides in length [14,15,16]. In terms of its association with cancer, MALAT1 has been shown to be oncogenic and to be overexpressed in several solid tumors including lung, colorectal, bladder and laryngeal cancers [24,25,26,27]
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