Expression and functions of long non-coding RNA MALAT1 during acute ischemia reperfusion kidney injury in rats

Abstract

Objective To investigate the expression pattern of long non-coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) in renal tissues of rats with acute ischemia reperfusion kidney injury(AIKI)and its influence on AIKI. Methods 128 clean grade rats were randomly divided into 3 groups including the control, model and experimental group. Rats in model and experimental groups were established into AIKI model according to the international standard, and rats in the experimental group were additionally injected with 5 mg/kg MALAT1 siRNA every day. The expression and distribution of MALAT1 in renal tissues at 2 h, 24 h, 48 h, 72 h, day 7 and day 14 were detected by real-time quantitative PCR. At 48 h and 72 h after operation, serum biochemical parameters, urine volume and urine composition were analyzed. Results MALAT1 was mainly expressed in the cortex of normal kidney tissues. Response to AIKI, the expression of MALAT1 robustly increased, reached the peak at 48 h, decreased significantly at 72 h, and recovered to the normal level on day 14. The level of MALAT1 was significantly lowered by the injection of MALAT1 siRNA.The levels of serum urea nitrogen, creatinine, urine protein and urine ketone were significantly reduced by down regulation of MALAT1. Conclusion The expression of MALAT1 is significantly increased in renal tissue of AIKI rats, and blockade of MALAT1 can alleviate acute ischemia reperfusion kidney injury in vivo. Key words: Long non-coding RNA-MALAT1; Acute ischemia reperfusion kidney injury; Gene interference