Maize–Fusarium associations and their mycotoxins: Insights from South Africa

Abstract

For maize, a staple food in South Africa, there is a lack of comprehensive knowledge on the mycotoxin-producing fungal diversity. In this study, a fungal community profile was established using culture-dependent methods for 56 maize seed samples that were also analysed for 13 mycotoxins. The fungal isolates were identified by morphology and DNA sequencing. A total of 723 fungal isolates from 21 genera and 99 species were obtained and characterised. Fusarium was the most common genus (isolated from 52 samples), followed by Cladosporium (n = 45), Aspergillus (n = 41), Talaromyces (n = 40), and Penicillium (n = 38). Fusarium communities were dominated by the Fusarium fujikuroi species complex, which includes species such as Fusarium verticillioides and Fusarium temperatum, while Fusarium awaxy and Fusarium mirum are reported here for the first time from South Africa. As for the deoxynivalenol (DON) producing species, only Fusarium boothii and Fusarium graminearum were isolated to a lesser extent. DON (n = 37), fumonisins (FUM) (n = 32), and zearalenone (ZEA) (n = 6) were detected. The presence of a particular species did not guarantee the presence of the corresponding mycotoxins, while the inverse was also true. The occurrence of DON and/or FUM in South African maize remains a health concern, so continuous monitoring of both fungal species and their mycotoxins is important.