Abstract
Maize stalk rot caused by Fusarium species is one of the most important fungal diseases of maize throughout the world. The disease is responsible for considerable yield losses and has also been associated with mycotoxin contamination of the crop. In this study, a survey of maize stalk rot was performed in seven locations of Yunnan Province in China during the cropping season of 2015 and 2016. Based on morphological and molecular characteristics, 204 isolates belonging to 12 Fusarium spp. from symptomatic stalks of maize were identified. Among the isolated strains, 83 were identified as Fusarium meridionale (40.5%), 46 as Fusarium boothii (22.5%), 34 as Fusarium temperatum (16.5%), 12 as Fusarium equiseti (5.9%), 10 as Fusarium asiaticum (4.9%), six as Fusarium proliferatum (3.0%), four as Fusarium verticillioides (2.0%), four as Fusarium incarnatum (2.0%), two as Fusarium avenaceum (1.0%), one as Fusarium cerealis (0.5%), one as Fusarium graminearum (0.5%), and one as Fusarium cortaderiae (0.5%). Fusarium cortaderiae was the first report on the causal agent of maize stalk rot disease in China. These isolates were divided into five chemotypes: nivalenol (NIV), deoxynivalenol (DON), beauvericin (BEA), zearalenone (ZEN), and fumonisin (FUM). Phylogenetic analysis based on partial sequences of the translation elongation factor 1α (TEF1-α) showed a high degree of interspecific polymorphisms among the isolates. Pathogenicity analysis on maize stalks indicated that all the 12 species of Fusarium were able to cause the disease symptoms with different aggressiveness. This study on population, pathogenicity, and toxigenic chemotypes of Fusarium species associated with maize stalk rot in Yunnan Province of southwest China, will help design an effective integrated control strategy for this disease.
Highlights
In the Yunnan Province of southwest China, maize plays a crucial role in local agricultural production
Twelve Fusarium species were found including, 83 isolates as F. meridionale (40.5%), 46 isolates were F. boothii (22.5%), 34 isolates identified as F. temperatum (16.5%), 12 as F. equiseti (5.9%), 10 isolates were F. asiaticum (4.9%), six were F. proliferatum (3.0%), four as F. verticillioides (2.0%), four as F. incarnatum (2.0%), two as F. avenaceum (1.0%), one as F. cerealis (0.5%), one as F. graminearum (0.5%), and one as F. cortaderiae (0.5%; Figure 1)
F. meridionale, F. boothii, F. asiaticum, F. cortaderiae, and F. cerealis belonged to the Fusarium sambucinum species complex (FSAMSC) and shared similar morphological characteristics
Summary
In the Yunnan Province of southwest China, maize plays a crucial role in local agricultural production. Fusarium is an important plant pathogenic fungus with a wide range of hosts, including corn, wheat, rice, and other cereal crops (Boutigny et al, 2011) These pathogens cause ear and stalk rot disease, potentially damaging to crop yield and food safety. Fusarium temperatum is an important maize pathogen and described as a new species causing disease in maize crop (Scauflaire et al, 2011) These pathogens can produce different toxigenic chemotypes, demonstrating the tremendous potential of this species for mycotoxin contamination (Duan et al, 2016). Many Fusarium species have been reported to responsible for maize ear and stalk rot disease in China, no detailed studies have been done in Yunnan Province based on composition, pathogenicity, and toxigenic chemotypes
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