Abstract
The effect of serial passage in immune-deprived mice on certain biological and biochemical parameters has been studied in a series of 6 human colorectal tumour xenografts. Histological integrity is maintained for up to 10 serial passages, together with production of epithelial mucins and carcinoembryonic antigen. Passaged tumours retain human lactate dehydrogenase and glucose-6-phosphate dehydrogenase isoenzyme patterns and a human chromosome constitution. The induction of a murine tumour has been identified in this system, and the importance of routine checks for the presence of human tissue during serial passage is stressed.
Highlights
Summary.-The effect of serial passage in immune-deprived mice on certain biological and biochemical parameters has been studied in a series of 6 human colorectal tumour xenografts
Before the xenograft model may be regarded as a useful predictive screen for new chemotherapeutic agents, or as a relevant model for the study of the effectiveness of existing compounds, data are required on whether tumours, after prolonged serial passage, retain the characteristics of the human primary from which they were derived
Fibrotic material observed in sections from the human primary tumours at surgical resection was not observed in xenograft tumours
Summary
Summary.-The effect of serial passage in immune-deprived mice on certain biological and biochemical parameters has been studied in a series of 6 human colorectal tumour xenografts. THERE have been many reports in the literature on the successful growth and maintenance of human colorectal tumours in immune-deprived mice (Houghton and Taylor, 1976; Cobb, 1973; Pickard, Cobb and Steel, 1975) and in nude mice (Povlsen and Rygaard, 1971). Before the xenograft model may be regarded as a useful predictive screen for new chemotherapeutic agents, or as a relevant model for the study of the effectiveness of existing compounds, data are required on whether tumours, after prolonged serial passage, retain the characteristics of the human primary from which they were derived. The current report presents data on some biological and biochemical characteristics of human colorectal tumour xenografts during serial passage in both male and female immunedeprived mice
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