Abstract
The key point of transport energization across the plasma and vacuolar membrane of fungi is the generation of the electrochemical gradient of protons by H+-ATPases of both membranes. The gradient of K+ is used to increase the energy capacity of the H+ gradient. The regulation of ion concentrations in the yeast cytosol is based on the activity of transporters of plasmalemma and tonoplast. This chapter discusses the transport of Mn2+ or other divalent cations (Me2+). Mn2+ uptake by yeasts is an energy-dependent process. It is driven by the H+ gradient both in the presence and in the absence of glucose: protonophores inhibit the accumulation of Mn2+ and Co2+. The dissipation of the K+ gradient by different reagents decreases the Mn2+ uptake. Candicydin brings about a rapid and complete loss of the intracellular K+ and inhibits Mn2+ uptake. The inhibition of Mn2+ uptake by reagents collapsing the K+ and H+ gradients point to the participation of these gradients in the energization of the Mn2+ uptake. The chapter presents results that support the electrogenic efflux of K+.
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