Abstract
Ochratoxins are possible human carcinogens. The aim of this study is to develop a sensitive competitive immunofluorescent analysis for determination of ochratoxin A (OTA) on the base of immobilized polyclonal antibody against ochratoxin and F(ab)2 fragment on magnetic nanoparticles (MNPs). Competitive immunoassay was performed by using variety concentrations of OTA and constant concentration of fluorescein isothiocyanate (FITC) labeled OTA. The analytical characteristics of the analysis with immobilized polyclonal antibody and F(ab)2 fragment were very closely.
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