Abstract
ABSTRACTPurification is one of the most important steps in biotechnological downstream process. This procedure is associated with some difficulties including several separation steps. Common cell separation procedures are usually time and energy consuming that also compromises cell growth, viability and reusability. Magnetic immobilization was introduced as a novel separation technique to provide a quick, easy and convenient alternative vs. traditional cell harvesting methods. In this study, recombinant E. coli producing extracellular asparaginase was decorated by synthesized L-lysine coated iron oxide nanoparticles and cell growth, immobilization efficiency and asparaginase productivity was evaluated.
Published Version
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