Abstract
The covalent bionic modifications of hydrothermal carbonaceous nanomaterials (HCNs) are of great potential in extraction and separation engineering because they can avoid present disadvantages of chemical functionalization and achieve selective recognition capacity with stability and sustainability. This article developed novel dual functionalized HCNs with sealed magnetic nanospheres (MNs) and cell membranes (CMs) of osteoblast, via the hydrothermal carbonization of glucose on MN surface and the aqueous amidation between terminal amino groups on CMs and surface carboxyl groups on HCNs. Owing to the specific combinations between drugs and membrane receptors, CMs bonded magnetic HCNs (CM-MHCNs) were applied as a drug discovery platform to screen potential anti-osteoporosis components from traditional herb medicine. The introduction of hydrothermal carbonaceous layer on MNs was proved to sharply improve their water dispersibility, resistance to oxidation and acids and CM grafting capacity with good magnetic property. These combined with the covalent bonding between prepared MHCNs and CMs provided the enhanced stability, where the loss amount (7.0 mg g−1) was dramatically reduced in comparison with that of CMs adsorbed MHCNs (17.9 mg g−1) and MNs (18.7 mg g−1). The resultant CM-MHCNs showed high adsorption ability, rapid binding kinetics, good selectivity and reusability. Consequently, two novel potential anti-osteoporosis compounds: isopimpinellin and xanthotoxol were successfully discovered from Common Cnidium Fruit, which were confirmed by pharmacodynamic experiments. Their possible membrane receptors were further investigated by molecular docking and western blot analysis. These researches on CM-MHCNs offer a deep insight into green design of stable biomaterials and show their promising prospect in the discovery of drug leads, thus promoting the development of traditional herb medicines.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
